Glucoamylase enzyme for use in saccharification and fermentation

Description

Glucoamyl L-209 is a purified native glucoamylase produced from Aspergillus niger that functions by the hydrolysis of terminal 1, 4 linked alpha-D-glucose residues from non-reducing ends of polysaccharide chains with release of beta-D-glucose. Its application is to yield monomeric glucose molecules that can be fermented by yeast into biofuels of interest such as ethanol.


Typical Characteristics

Activity:165,000 u/mL (minimum)
Appearance:Brown liquid
pH:3.5 – 5.0
Bulk Density:1.11 – 1.16 g/mL

Unit Definition

One unit of glucoamylase (u) is the amount of enzyme which hydrolyzes soluble starch to liberate 1 milligram of glucose per hour under the conditions of the assay. A detailed assay method is available upon request.


Performance Benefits

Glucoamyl L-209 provides the following benefits to ethanol producers.

  • Robust and consistent performance under varied conditions
  • Enzyme conversion kinetics matches more closely to yeast growth curve
  • Increased alcohol concentrations
  • Lower residual sugars at end of fermentation
  • Reduced glycerol concentrations throughout fermentation

 

Application Recommendations

Glucoamyl L-209 is used to saccharify liquefied mashes of whole ground corn. The resultant glucose is fermented by yeast to produce ethanol.

Glucoamyl L-209 has a broad operating range for enhanced process flexibility and performs well within the temperature and pH ranges typical in whole ground corn batch fermentations.

In high gravity fermentations, performance can be further enhanced by the addition of SZM XT-20 fungal alpha amylase and/or SZM AP-1 acid protease.

 

Dosage Recommendation

Glucoamyl L-209 should be dosed at 0.051 - 0.068% weight enzyme / weight as is corn. The actual dose required will depend upon the conditions of your fermentation: time, initial pH and the level of solids.


Regulatory Status

  • ISO 9001 registered company
  • Non – GMO
  • Kosher certified
  • GRAS (Generally Recognized as Safe)
  • Animal feed approved (AAFCO Table 30.1)
  • In compliance with the Toxic Substances Control Act (TSCA)


Packaging

Glucoamyl L-209 is available in 1,125 kg totes or 20,250 kg bulk tankers.


Storage

Glucoamyl L-209 will meet the declared activity upon arrival at your plant. Glucoamyl L-209 can be safely stored in sealed containers. Enzymes should be stored below 770F (250C) and sheltered from direct sunlight for maximum stability.

 

 

Shelf Life

The enzymatic activity of Glucoamyl L-209 remains at ≥ 95% for six months when stored at 25oC. The recommended dosage may need to be increased if stored for longer than six months.


Safety and Enzyme Handling

Glucoamyl L-209 contains proteins obtained from large-scale cultures of the mold Aspergillus niger. Proteins may cause an allergic-type reaction in sensitized individuals that could require immediate medical attention. Prolonged direct contact may cause minor irritation to skin, eyes, or mucus membranes. For detailed handling information, please refer to the appropriate Material Safety Data Sheet.


Technical Service

CTE Global, Inc. is committed to formulating a productive and mutually beneficial relationship with ethanol producers in order to make their fermentation process as consistent, efficient, and cost-effective as possible. More detailed information about the application of this product is available upon request. If you have any questions, please contact us and let us know how we can be of assistance.


For more information contact:


CTE Global, Inc.

40 Skokie Boulevard, Suite 460

Northbrook, IL 60062 USA

Telephone: 847-564-5770

Fax: 847-272-6003

Web address: www.cte-global.com